Dh5 alpha subcloning efficiency
WebAug 2, 2024 · This product has been discontinued. Alternatives are NEB® 10-beta Electrocompetent E. coli ( NEB #C3020K) or chemically competent cells, NEB® 5-alpha Competent E. coli (High Efficiency) ( NEB #C2987) Catalog # C2989 was discontinued on August 02, 2024. NEB 5-alpha Electrocompetent Competent E. coli is a derivative of the … WebDH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, …
Dh5 alpha subcloning efficiency
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Webtransformation efficiency. The stock solution of pFastBac™-gus (0.2 g/ml), µ provided with pFastBac™1 Expression Vector (Cat. No. 10360-014), can be used as a control for the transposition frequency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. 1. WebSubcloning Efficiency™ DH5α™ Chemically Competent E. coli Catalog no. 18265-017 General Information and Kit Contents Shipping and Storage The kit is shipped on dry ice. Upon receipt, store the kit at -70°C. Do not store in liquid nitrogen. Kit Contents The kit contains the following reagents. Reagent Composition Amount
WebE. coli. (High Efficiency) NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide variety of applications. Available in a wide variety of sizes, including single-use vials, 200 μl vials, 96- and 384- well ... WebThermo Scientific™ DH5α Competent Cells. Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. Brand: Thermo Scientific™ EC0111. Additional Details : Weight : 17.00000kg. Product Code. 16506900. £50.25 / 40 reactions.
WebDH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three mutations: recA1, endA1 … WebDH5 is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5 increase insert stability and imp ...
WebMar 16, 2024 · Economical for routine subcloning procedures or applications where starting DNA is not limiting. Brand: Invitrogen™ 18265017. Additional Details : Weight : …
WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5 … iowa high school state wrestling streamingWebThermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of … iowa high school state wrestling 2023 winnersWebDH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications: DNA cloning following PCR or restriction digestion reactions. Subcloning (transferring DNA from a parent vector to a destination vector) Plasmid isolation. Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 … iowa high school state wrestling meetWebSubcloning Efficiency™ DH5α™ Competent Cells are tested for transformation efficiency using the pUC19 control DNA supplied with the kit and using the protocol on page 2. Transformation efficiency should be greater than 1 x 106 transformants/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity, open arms health care clinicWebChemically competent E. coli cells suitable for subcloning efficiency transformations, such as plasmid transformation or routine subcloning (e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency of these cells is not sufficiently high for most other cloning applications. For that, we recommend NEB® 5-alpha Competent E. coli (High … iowa high school state wrestling liveWebDH5-alpha Electrocompetent E. coli cells are suitable for high efficiency transformation in plasmid isolation, cloning, subcloning and more. Mutations in endA1 and recA1 ensure increased plasmid yield and … iowa high school state wrestling scheduleopen arms home health care